Beauveria bassiana as a Biocontrol Agent Against the Red Imported Fire Ant
نویسندگان
چکیده
Major Accomplishments to date: 1. Genetic Manipulations. The fungal transformation expression vector, pBARGPE1 was provided by Dr. Krishna Podila, Michigan Technological University. The vector had the Aspergillus nidulans gpdA promoter and the A. nidulans trpC terminator flanking a multiple cloning site. The ß-glucuronidase gene (GUS) was provided by Dr. Randy Allen, Texas Tech University. The 1.8 kb GUS gene was excised by restriction enzyme digestion of pRTL2-GUS as a BamH1-EcoR1 fragment. The BamH1 site was blunt-ended using T4 DNA polymerase. Transformation vector pBARGPE1 was digested with Xho1, blunt-ended, and digested with EcoR1. The GUS gene was thus directionally cloned under control of the gpdA promoter using T4 DNA ligase. The resulting 7.3 kb plasmid construct was transformed into Escherichia coli DH5a using CaCl2 transformation.
منابع مشابه
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